Vol. 10, Issue 1 (2021)

Author(s):
Surender R, Suneetha K, Revathi PR, Aneela M, Chiranjeevi M, Nagaraju P and LV Subba Rao

Abstract:
To study the identification of restorers with different molecular markers which are linked to fertility restorer genes Rf3 and Rf4 of WA-CMS system, 43 parental lines were screened for fertility restoration with the help of molecular markers linked to major fertility genes Rf3 and Rf4. The SSR primer RM6100 linked to Rf4 gene on chromosome 10 and SF 21-5 linked to Rf3 gene on chromosome 1 are used efficiently for identifying restorer lines in crop improvement programmes. Therefore, these markers are useful tool for evaluating large number of breeding lines to know about their fertility restoration in a short period of time without generating and evaluating large number of test crosses. Ten potential restorers identified (Rf4 and Rf3 present) with hundred percentage efficiency based on molecular screening, if candidate genes based markers are developed and validated for both Rf4 and Rf3 genes.