Vol. 10, Issue 1 (2021)

Author(s):
VB Thange, AB Bagade and MP Wankhade

Abstract:
A comparative study of frequency and spectrum of chlorophyll mutations induced by physical (gamma rays) and chemical mutagens (EMS) in relation to the effect of mutations in M₂ generation plant of kharif sorghum (Sorghum bicolor L.) genotype ‘PVK 801’. The experimental material comprised of different mutagenic treatments treated with different concentrations viz., 0.1%, 0.2%, 0.3% were EMS treatments and 10 kR + 0.1% EMS, 20 kR + 0.1% EMS, 30 kR + 0.1% EMS and 40 kR + 0.1% EMS were combination treatments and dry and wet control treatments. All these treatments were grown in randomized block design with three replications during kharif 2019. Results showed induction of broad spectrum of chlorophyll mutations which included albino, xantha, chlorina, viridis and xanthaviridis. Among these albino type was predominant in all the mutagenic treatments. Highest frequency of chlorophyll mutants were found under 20 kR + 0.1% EMS combination treatment. Based on the chlorophyll mutation frequency and spectrum in combination treatment were most effective followed by EMS treatments. The frequencies and spectrum of five different kind of induced chlorophyll mutations was in the order; albino > xantha > chlorina = viridis = xanthaviridis.