Purpose: Pippalyadi Yoga is a combination of four drugs mentioned in Bhaishajya Ratnavali Strirogadhikara for strivandyatwa which is used in the form of curna. This work was carried out to standardize all the ingredients of Pippalyadi Yoga.
Methodology: Curna of Pippali, Maricha Shunti and Nagakesara are prepared, mixed together and stored in an airtight bottle. Analysis of the Pippalyadi yoga was done with following parameters physico-chemical analysis like pH, refractive index, specific gravity, total solids, viscosity and chromatographical analysis like High performance thin layer chromatography (HPTLC).
Result: Loss on drying value of trikatu curna (TC) is 12.87 % w/w and 10.74%w/w of nagakesara. Ash value of trikatu curna is 5.33% w/w and 11.79 % w/w of nagakesara. Acid insoluble ash value is 0.29 %w/w of TC and 7.93% w/w of nagakesara. Water soluble ash values are 2.89% w/w of TC and 1.01 % w/w of nagakesara. Water soluble extractive values are 14.94 of TC and 4.64% w/w of Nagekesara and alcohol soluble extractive values are 6.07 of TC and 10.90 % w/w of Nagakesara. HPTLC of Trikatu curna: 7 prominent spots with Rf values 0.01, 0.04, 0.10, 0.25, 0.32, 0.50, 0.60 at 254nm and 5 spots with Rf values 0.01, 0.10, 0.36, 0.61, 0.83 at 366 nm. Nagekesara curna: 10 prominent spots with Rf values 0.00, 0.03, 0.17, 0.36, 0.52, 0.59 0.65, 0.72, 0.86, 0.92 at 254nm. 9 spots with Rf values 0.01, 0.04, 0.09, 0.34, 0.46, 0.55, 0.61, 0.68, 0.81 at 366nm.
Conclusion: The analytical data generated here may be considered for the development of standard parameters for the formulation.