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Vol. 9, Issue 9 (2020)

Comparison of parthenogenetic chemical activation of sheep prepubertal oocytes in Telangana

Author(s):
Ramsingh L, KRC Reddy and Gopala Reddy A
Abstract:
Parthenogenetic embryos were produced by using different concentrations of calcium ionophore + 6-dimethyl aminopurine (6-DMAP) in the concentrations of 1 µg/ml + 1 mM, 5 µg/ml + 2 mM and 10 µg/ml + 5 mM in protocol I, II and III. respectively and also a control was maintained using TCM 199 B + synthetic oviduct fluid (SOF). The results of embryonic development showed that there was significant (P<0.05) difference in cleavage rate between the activation treatments. Treatment II resulted in significantly (P<0.05) higher cleavage rate (66 ± 0.37%) followed by in vitro fertilization (IVF) (57.5 ± 0.38%), Treatment III (46.75 ± 0.62%) and Treatment I (38.5 ± 0.41%). No significant difference was observed between Treatment II and IVF pertaining to attainment of 2-cell stage, but differed significantly (P<0.05) when compared to Treatments I (21.75 ± 0.24%) and III (27.5 ± 0.32%). Pertaining to attainment of 4-cell stage, Treatment II resulted in significantly higher rate (30.25 ± 0.32%) followed by in-vitro fertilization (27.25 ±0.28%), Treatment III (23 ± 0.36%) and Treatment I (16.5 ± 0.18%). Pertaining to attainment of 8-cell stage, Treatment II resulted in significantly higher rate (26.75 ± 0.33%) followed by in-vitro fertilization (24.5± 0.23%), Treatment III (13.25± 3.20%) and Treatment I (15 ± 0.19%). Pertaining to attainment of Morula stage, Treatment II resulted in significantly higher rate (16.5 ± 0.23%) followed by in-vitro fertilization (14 ± 0.16%), Treatments III (12 ± 0.20%) and I (9.5± 0.16%).
Based on the results of the present study, it can be concluded that the exposure of in vitro mature oocytes to calcium ionophore at a concentration of 5 µg/ml for 5 min followed by 3 hrs incubation with 2 mM 6-DMAP (Protocol-II) was found to be the best for parthenogenetic embryo production in prepubertal ovine ovaries. Comparison of 2-cell stage between treatment II and IVF was similar. Other stages (4-cell, 8-cell and Morula stage) were significantly (P<0.05) higher in parthenogenetic activation of oocytes.
Pages: 220-222  |  444 Views  49 Downloads


The Pharma Innovation Journal
How to cite this article:
Ramsingh L, KRC Reddy, Gopala Reddy A. Comparison of parthenogenetic chemical activation of sheep prepubertal oocytes in Telangana. Pharma Innovation 2020;9(9):220-222.

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