Vol. 9, Issue 9 (2020)

Author(s):
Ramsingh L, KRC Reddy and Gopala Reddy A

Abstract:
Parthenogenetic embryos were produced by using different concentrations of calcium ionophore + 6-dimethyl aminopurine (6-DMAP) in the concentrations of 1 µg/ml + 1 mM, 5 µg/ml + 2 mM and 10 µg/ml + 5 mM in protocol I, II and III. respectively and also a control was maintained using TCM 199 B + synthetic oviduct fluid (SOF). The results of embryonic development showed that there was significant (P<0.05) difference in cleavage rate between the activation treatments. Treatment II resulted in significantly (P<0.05) higher cleavage rate (66 ± 0.37%) followed by in vitro fertilization (IVF) (57.5 ± 0.38%), Treatment III (46.75 ± 0.62%) and Treatment I (38.5 ± 0.41%). No significant difference was observed between Treatment II and IVF pertaining to attainment of 2-cell stage, but differed significantly (P<0.05) when compared to Treatments I (21.75 ± 0.24%) and III (27.5 ± 0.32%). Pertaining to attainment of 4-cell stage, Treatment II resulted in significantly higher rate (30.25 ± 0.32%) followed by in-vitro fertilization (27.25 ±0.28%), Treatment III (23 ± 0.36%) and Treatment I (16.5 ± 0.18%). Pertaining to attainment of 8-cell stage, Treatment II resulted in significantly higher rate (26.75 ± 0.33%) followed by in-vitro fertilization (24.5± 0.23%), Treatment III (13.25± 3.20%) and Treatment I (15 ± 0.19%). Pertaining to attainment of Morula stage, Treatment II resulted in significantly higher rate (16.5 ± 0.23%) followed by in-vitro fertilization (14 ± 0.16%), Treatments III (12 ± 0.20%) and I (9.5± 0.16%).