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Vol. 9, Issue 6 (2020)

Validated ultra-high performance liquid Chromatographic method for estimation of Atenolol in bulk powder and Pharmaceutical dosage forms

Author(s):
Palled Mahesh Shivbasappa, Shailendra Suryawanshi Sanjay, Siddhant Mardolkar, Mahendra Kumar chauhan and Sunil Satyappa Jalalpure
Abstract:
The objective of proposed research work was to develop and validate new simple, selective, specific, accurate, precise and robust Ultra High Performance liquid chromatographic (UHPLC) method for estimation of Atenolol in bulk and pharmaceutical products. Method was developed by utilizing mobile phase composed of methanol: 0.1% orthophosphoric acid (50:50 v/v) with the use of BDS Hypersil C18(250 × 4.6 mm, 5 µm) column as stationary phase. The flow rate of mobile phase was 1.5ml/min and the temperature was maintained at 20 oC. Atenolol was detected at 224nm using UV detector. The retention time of Atenolol was found to be 8.9 min. It showed linear response between the concentration ranges 4-8 µg/ml with correlation coefficient 0.999. The method was validated as per current ICH guidelines in terms of specificity, selectivity, linearity and range, precision, accuracy and robustness and all the values of validation was found to be within the acceptance. Hence the method can be used for the routine quality control evaluation of Atenolol in bulk powder.
Pages: 320-323  |  400 Views  23 Downloads


The Pharma Innovation Journal
How to cite this article:
Palled Mahesh Shivbasappa, Shailendra Suryawanshi Sanjay, Siddhant Mardolkar, Mahendra Kumar chauhan, Sunil Satyappa Jalalpure. Validated ultra-high performance liquid Chromatographic method for estimation of Atenolol in bulk powder and Pharmaceutical dosage forms. Pharma Innovation 2020;9(6):320-323.
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