Vol. 9, Issue 5 (2020)

Author(s):
Ankita Kumari, Abhishek Gaurav, Nirmal Kumar and Hitesh Kumar

Abstract:
This study was aimed at the molecular characterization of Staphylococcus aureus isolated from milk samples. A total of 100 milk samples comprising of pooled milk (n=25), vendors milk (n=25), pasteurized market milk (n=25) and individual cow milk (n=25) were collected during from Udaipur city, Rajasthan. The S. aureus were found in g pooled milk, vendors milk, pasteurized market milk and individual cow milk as 76%, 36%, 0% and 56%, respectively. Out of total 42 S. aureus isolates, 10 were multidrug resistant which were further subjected for molecular characterization by PCR. All the 10 isolates were found positive for 16S rRNA and nuc gene. The prevalence of antibiotic resistant genes mecA, ermC and aacA-aphD were 40% (4), 70% (7) and 30% (3), respectively.