Isolation of bovine rotavirus in MDBK cell line from diarrhoeic calves of Navsari district
Pushpa M Makwana, IH Kalyani and Dhruv Desai
The present study was designed to study isolation of rotavirus from faecal samples of bovine calves and to observe cytopathic effects of rotavirus. For this, total 157 faecal samples comprising 104 cattle and 53 buffalo calves were aseptically collected. Samples found positive by Latex Agglutination Test (LAT) were used for further study using MDBK cell line. By LAT 17 samples were found positive and these samples were used for cell culture study. All the 17 samples were inoculated in MDBK cell line and from these 16 samples could be successfully isolated, adapted and showed characteristic cytopathic effects. Out of 16 samples 4 samples showed cytopathic effect in first passage, 7 samples showed CPE after 2nd passage and 5 samples showed cytopathic effects at 4th passage level. The characteristic cytopathic effects includes rounding of cells, spindle shaped morphology and disruption of normal monolayer. The conformation of virus was checked by VP6 gene based reverse transcriptase PCR (RT-PCR).
How to cite this article:
Pushpa M Makwana, IH Kalyani, Dhruv Desai. Isolation of bovine rotavirus in MDBK cell line from diarrhoeic calves of Navsari district. Pharma Innovation 2020;9(5):222-225.