Vol. 9, Issue 5 (2020)

Author(s):
C Sworna Kumari, TV Meenambigai, A Mangalagowri, A Palanisamy and G Sarathchandra

Abstract:
The VP2 protein of highly virulent infectious bursal disease virus (hvIBDV) was cloned and expressed in pRSET A vector with high level of protein expression. . VP2 is the host protective protein with major neutralizing epitopes was cloned and expressed in E. coli expression system BL21- DE3. The protein expression was evaluated by induction studies with IPTg and characterized by SDS PAGE and western blotting analysis. Thus, the present study focusing on expression of the immunodominant VP2 protein that may be used as a protein boost in DNA vaccine production strategy for enhanced immunity against IBDV infection in chickens in the near future.