Abstract:The aim of the study was to prepare and evaluate the efficacy of auto vaccine prepared against
Staphylococcol mastitis causing of
S. aureus. Using local isolates of the selected areas in and around Tirupati, Chittoor District within smaller groups of dairy cows. Whole cell
S. aureus auto vaccine was prepared for selected therapeutic areas in and around Tirupati, Chittoor District using local isolates. The evaluation of auto vaccine was carried out against mastitis of
S. aureus origin in eliminating
S. aureus from milk of 27 cows with subclinical mastitis. The antibiogram results of
S. aureus isolates in Chittoor district shown higher sensitivity to beta-lactam antibiotics like Amoxycillin (60.37%) followed by Ampicillin (56.60%) whereas in selected therapeutic areas higher sensitivity to
S. aureus isolates was observed to quinolones like Enrofloxacin (66.66%) and Ciprofloxacin (59.25%). Enrofloxacin was selected as antibiotics of choice based on antibiotic susceptibility test of
S. aureus isolates from therapeutic areas. The therapeutic trials were conducted in three divided groups of 9 cows each, group-I was given with antibiotic enrofloxacin, group-II was given with
S. aureus auto vaccine and group-III was given with both enrofloxacin and
S. aureus auto vaccine.
The antibiotic was administered parenterally, whereas auto vaccine was given subcutaneously nearer to the upper udder lymphnode region. After 35 days of the treatment S. aureus was not detected in the milk of 33.3% of the cows treated with enrofloxacin, 66.7% of the cows treated with S. aureus auto vaccine, and 100% of the cows that underwent combined therapy of Enrofloxacin and auto vaccine. The study results shown that combined therapy of antibiotic and auto vaccine was more effective in eliminating S. aureus from milk of S. aureus infected cows compared to either of them alone.