Vol. 9, Issue 5 (2020)
Diagnosis of canine trypanosomosis by wet blood film examination and staining techniques
Pooja Sree Alli, M Udaya Kumar, GS Sreenivasamurthy, P Kalyani and Sameena
In our present study different traditional parasitological techniques i.e., Wet Blood Film Examination and conventional staining techniques like Giemsa stain, Leishman stain, Field’s stain and Acridine orange staining technique were used for the diagnosis of canine trypanosomosis in the field. Out of 302 samples examined wet blood film examination revealed only one dog (0.33%) to be positive for trypanosomosis. Only two blood smears collected from 2 different dogs were found positive by all conventional staining techniques viz., Giemsa staining 0.66% (2/302), Leishman staining 0.66% (2/302), Field’s staining 0.66% (2/302) and Acridine orange staining 0.66% (2/302) techniques. The main aim was to study the prevalence of canine trypanosomosis because most of the cases go unnoticed without proper diagnosis. The control of these cases is essential, as these act as carriers to other animals and in rare cases to humans.
How to cite this article:
Pooja Sree Alli, M Udaya Kumar, GS Sreenivasamurthy, P Kalyani, Sameena. Diagnosis of canine trypanosomosis by wet blood film examination and staining techniques. Pharma Innovation 2020;9(5):136-138.