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Vol. 9, Issue 4 (2020)

Validated RP-HPLC method for simultaneous estimation of aclidinium and formoterol in bulk drug and dosage form

Author(s):
Shyamala, G Sai Kala, A Sravani, T Anusha and K Srikanth
Abstract:
A simple, Accurate, precise method was developed for the simultaneous estimation of the Aclidinium and formoterol in Pharmaceutical dosage form. Chromatogram was run through Standard Azilent 150 x 4.6 mm, 5µ. Mobile phase containing Buffer potassium dihydrogen phosphate: Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was potassium dihydrogen phosphate buffer. Retention time of Aclidinium and Formoterol were found to be 2.221 min and 2.801 min. %RSD of the Aclidinium and Formoterol were and found to be 0.9 and 1.2 respectively. %Recovery was obtained as 100.41% and 100.57% for Aclidinium and Formoterol respectively. LOD, LOQ values obtained from regression equations of Aclidinium and Formoterol were 0.33, 1.0 and 0.04, 0.12 respectively. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Pages: 242-247  |  196 Views  0 Downloads
How to cite this article:
Shyamala, G Sai Kala, A Sravani, T Anusha, K Srikanth. Validated RP-HPLC method for simultaneous estimation of aclidinium and formoterol in bulk drug and dosage form. Pharma Innovation 2020;9(4):242-247.
The Pharma Innovation Journal