Abstract: Gastroenteritis in dogs comprises of multiple etiologies which includes infectious and non-infectious causes. Amongst all the factors viral gastroenteritis is the most common and major problem. Canine parvovirus (CPV) infection is the most pathogenic viral infection of dogs causing haemorrhagic gastroenteritis and myocarditis in pups. This study was planned to compare haemato-biochemical parameters in CPV infected and non CPV infected dogs which included a total of thirty six dogs which were diagnosed with polymerase chain reaction for the detection of CPV in faecal samples. CPV positive dogs showed decreased haemoglobin values with increased packed cell volume, leucocytosis, and neutrophilia with thrombocytopenia. CPV positive dogs also showed elevated levels of liver and kidney function parameters such as ALT, AST, BUN, total protein and serum creatinine. Serum levels of sodium, potassium and chloride were found to be decreased in the affected dogs. Five days of therapy lead to restoration of the clinical parameters in normal range.