Erythropoietin prevents sepsis -induced clinical severity and haematological changes in mice
K Kannan, S Choudhury, T Ramasamy, M Kesavan, S Parida and TU Singh
We investigated the effect of erythropoietin on sepsis-induced haematological changes in mice. Sepsis was induced by caecal ligation and puncture. In this study, blood was collected after 20 ± 2 hr of surgery from Sham operated (SO), Sham operated (SO)+EPO, sepsis (CLP) and CLP+EPO. Clinical scoring was done. Septic animal showed bloated appearance, mucky eyes, lack of movement and lack of alertness. Erythropoietin treatment reduced the clinical severity in septic mice. Sepsis significantly increased haemoglobin, packed cell volume and decreased red blood cell count, white blood count and platelet count. Erythropoietin significantly reversed the haematological changes to near normal. However erythropoietin post treatment showed no significant change in white blood and platelet count. Erythrocyte indices did not reveal any changes in sepsis except MCH (Mean Corpuscular haemoglobin) which was increased. In septic mice treated with erythropoietin, significant decrease in MCH was observed. Mean platelet volume, red cell distribution width and platelet distribution widths were significantly increased in sepsis. Erythropoietin restored the red cell distribution width compared to control, but no significant change was observed in mean platelet volume and platelet distribution width in septic mice. In conclusion, erythropoietin prevents sepsis-induced haematological changes in mice, but not platelet parameters.
How to cite this article:
K Kannan, S Choudhury, T Ramasamy, M Kesavan, S Parida, TU Singh. Erythropoietin prevents sepsis -induced clinical severity and haematological changes in mice. Pharma Innovation 2020;9(2):81-84.