Vol. 9, Issue 11 (2020)
Plant bioregulators and its effect on growth and development of Kachai lemon (Citrus jambhiri Lush.) in-vitro
Lorinda Kangabam, Rocky Thokchom, Naoroibam Silpia Devi and Nigthoujam Sandhyarani Devi
In the present study, micropropagation of Kachai lemon was attempted through direct regeneration from micro-cuttings of the in-vitro germinated seedlings followed by rhizogenesis and subsequently acclimatization. MS medium supplemented with Kinetin (0.5 mg/l) and GA3 (1.0 mg/l) (T11) sprouted lemon seeds earlier (9.87 days). Highest microshoot length (9.55 cm) was observed in treatment T12 (MS+ 1.0 mg/l Kn + 1.0 mg/l GA3), while treatment T13 (MS+ 1.5 mg/l Kn + 1.0 mg/l GA3) produced maximum number of microshoots per culture (5.93) at 90 DAI. MS + 0.5 mg/l Kn produced maximum number of leaves per microshoot (8.73). Earlier root initiation (10.07 days) was observed in MS basal medium containing 0.5 mg/l IBA + 1.0 mg/l NAA (T9). Maximum root length (5.54 cm each) of Kachai lemon was observed in MS medium fortified with 0.5 mg/l IBA + 1.0 mg/l NAA (T9) and 1.25 mg/l IBA + 1.0 mg/l NAA (T11). Treatment T2 (MS + 0.25 mg/l IBA) produced maximum number of roots per microshoot while the rooting percentage was recorded maximum in treatment T13 (MS+ 2.0 mg/l IBA + 1.0 mg/l NAA). Least performance was shown by control (T1) in all the cases. Acclimatization of in vitro regenerated plantlets of Kachai lemon using vermiculite and vermicompost at equal proportion (1:1) showed earliest adaptation (H3) with better survivability rate (92.89%) whereas, use of equal proportion of soil and vermicompost showed inferior result (H5) with least plantlet survivability (57.39%).
How to cite this article:
Lorinda Kangabam, Rocky Thokchom, Naoroibam Silpia Devi, Nigthoujam Sandhyarani Devi. Plant bioregulators and its effect on growth and development of Kachai lemon (Citrus jambhiri Lush.) in-vitro. Pharma Innovation 2020;9(11):18-21.