Vol. 9, Issue 10 (2020)
Effect of capsaicin in inhibiting efflux pump AcrAB-Tol C of tetracycline resistant Escherichia coli
Nisha AR, Preethy John, Vanishree, V Keerthika and Arya Moha
Bovine mastitis has become extremely complex and costliest disease among the livestock diseases in India. The key factors for therapeutic failure in mastitis are the acquisition of antimicrobial resistance among bacteria. Multidrug efflux systems make a major contribution to the increased tolerance of bacteria to multiple antibiotics that strip antimicrobial agents from microbial cells and make them resistant to antimicrobials. Therefore, blocking the efflux pumps is one of the methods to circumvent antimicrobial resistance, thereby supplying bactericidal/static concentrations inside the cytoplasm. Among various class of efflux pump AcrAB-TolC efflux system are responsible for antimicrobial resistance in Escherichia coli (E.coli). In this study capsaicin, a phyto-alkaloid was used as modulator of efflux pump in combination with tetracycline in tetracycline resistant E.coli. Antimicrobial sensitivity carried out by using standard disc diffusion method with appropriate controls .Tube dilution method is carried out and the turbidity is measured at 600nm. mRNA expression for the respective genes is carried out by Real time PCR. There was no zone of inhibition for capsaicin and tetracycline when they were used alone, while there was significant increase in the zone of inhibition for tetracycline when it was combined with capsaicin. Turbidity of the culture decreases when tetracycline is used in combination with capsaicin. mRNA expression for AcrAB-TolC is decreased when tetracycline is used in combination with capsaicin indicating the blocking of efflux pumps. Use of phytochemicals like capsaicin, which is a known inhibitor of efflux pump, could interfere with efflux pumping of antibiotics in resistant bacteria. Thus combination of antibacterial agent with capsaicin can be a promising approach to combat antibiotic resistant bovine mastitis due to E.coli.
How to cite this article:
Nisha AR, Preethy John, Vanishree, V Keerthika, Arya Moha. Effect of capsaicin in inhibiting efflux pump AcrAB-Tol C of tetracycline resistant Escherichia coli. Pharma Innovation 2020;9(10):39-45.