Vol. 9, Issue 10 (2020)

Author(s):
Prateek Sharma, Arunabh Joshi, Suresh Rajoriya and Devendra Jain

Abstract:
Present investigation was carried out at department of molecular biology and biotechnology, Rajasthan College of Agriculture, Udaipur to determine the genetic variations in the most valuable medicinal crop Opium poppy (P. somniferum L.). Total 24 diverse accessions of Opium poppy were evaluated for genetic diversity assessment using RAPD (Random Amplified Polymorphic DNA) marker. 15 primers yielded 130 amplified loci, of which 108 were polymorphic. The polymorphism amongst 24 genotypes of P. somniferum was 83.07% and the overall size of PCR amplified products ranged between 100 bp to 2700 bp. The Jaccard’s similarity coefficient value 0.43 to 0.86 certified the variation distribution is diverse. UPGMA based phylogenetic tree depicting the genetic relationship among 24 opium poppy accessions could be grouped into two major clusters. These cluster further again classified into two sub-clusters. This RAPD assay provides significant heterogeneity information of opium poppy accessions for further exploitation in crop improvement.