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Vol. 9, Issue 1 (2020)

Determination of flavonoids and hydroxycinnamic acids in the herb of common agrimony by HPLC method

NM Huzio, AR Grytsyk, LI Budniak and IR Bekus
The creation of new herbal products and the improvement of their production technologies is an important area of pharmaceutical science. A valuable source of biologically active substances is a representative of the Rose family (Rosaceae) common agrimony (Agrimonia eupatoria L.). Remedies on the basis of common agrimony are used to increase the secretion of the digestive glands, as appetizing, choleretic, hemostatic, astringent, anti-diuretic and anti-inflammatory agent. The chemical composition of the common agrimony herb grown on the territory of Ukraine is poorly studied, so conducting an in-depth phytochemical study of the raw material of this type of plant is relevant. The purpose of the work was the determination of individual flavonoids and hydroxycinnamic acids by high performance liquid chromatography (HPLC). The determination of the qualitative composition and the quantitative content of the individual flavonoids and hydroxycinnamic acids in the common agrimony herb were performed by HPLC on an Agilent 1200 chromatograph.
As a result of the conducted studies, the quantitative content of 4 flavonoids – isoquercitrin (916.73 µg/g), neohesperidin (3850.93 µg/g), naringenin (308.28 µg/g) and luteolin (332.13 µg/g) was found and determined in the common agrimony herb. Among the hydroxycinnamic acids, hydroxyphenylacetate (1145.25 μg/g), caffeic (614.17 μg/g), syringic (215.86 μg/g), p-coumaric (827.54 μg/g), ferulic (267.72 μg/g), sinapic (381.35 μg/g), cinnamic (251.81 μg/g) and quinic (77.28 μg/g) acids were detected in the studied raw material. Neohesperidin, prevails among flavonoids in the common agrimony herb, hydroxyphenylacetate and p-coumaric – among the hydroxycinnamic acids.
Pages: 43-46  |  653 Views  26 Downloads

The Pharma Innovation Journal
How to cite this article:
NM Huzio, AR Grytsyk, LI Budniak, IR Bekus. Determination of flavonoids and hydroxycinnamic acids in the herb of common agrimony by HPLC method. Pharma Innovation 2020;9(1):43-46.
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