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Vol. 9, Issue 1 (2020)

Evaluation of shelf life of live attenuated lentogenic strain PPMV-1 vaccine

Author(s):
Raya Sarkar, Chanchal Guha, Ujjwal Biswas, Amaresh Chatterjee and Partha Sarathi Jani
Abstract:
The present study was conducted to evaluate the shelf life of live attenuated lentogenic strain PPMV-1 vaccine. Twenty four, 1 ml freeze dried vaccine vials were kept in the refrigerator at -120 0C temperature. Each freeze dried vial contained 0.5 ml allantoic fluid containing live attenuated lentogenic strain PPMV-1 vaccine virus (local isolate) with addition of 0.5 ml cryoprotectant. HA titer as and EID50 were estimated just before keeping in the storage (in refrigerator at -120 0C temperature) and thereafter, every 15 days interval until the viability of the vaccine virus became nil. before freeze drying of the live attenuated lentogenic strain PPMV-1 vaccine (local isolate) the HA titer, EID50 and number of dose of the vaccine per vial were 210, 1010.57 and 5874 respectively. After freeze drying, those values reduced to 210, 1010.37 and 3706.5 accordingly to HA titer, EID50 and number of dose of vaccine per vial respectively. After keeping of the vaccine at -120 0C temperature, the HA titer reduced to 29 that had remained unchanged, up to 90 days of post storage study period. On the other hand, the EID50 remained unchanged from the day of storage up to 90 days post storage study course at satisfied level. And, subsequently, the dose of the vaccine per vial also remained unchanged i.e. 3706.5 which was satisfactory up to the end of the study period. From the study it was concluded that freeze dried live attenuated lentogenic strain PPMV-1 vaccine (local isolate) had not lost its infectivity at -120 0C within 3 months.
Pages: 147-149  |  519 Views  2 Downloads


The Pharma Innovation Journal
How to cite this article:
Raya Sarkar, Chanchal Guha, Ujjwal Biswas, Amaresh Chatterjee, Partha Sarathi Jani. Evaluation of shelf life of live attenuated lentogenic strain PPMV-1 vaccine. Pharma Innovation 2020;9(1):147-149.
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