Vol. 8, Issue 9 (2019)

Author(s):
Kewat RR, Sharma RK, Gautam Vidhi, Shraman K, Jogi J and Dinodiya N

Abstract:
The present study was conducted to isolate and identify endophytic bacteria from leaves of Cymbopogon citratus (Lemongrass) and in vitro antibacterial activity was observed on Gram positive and Gram negative bacteria. Twenty leaves samples from Cymbopogon citratus were taken. The leaves were sterilized and incubated into King’s B agar medium and then again sub-cultured into blood agar and then transferred into BHI broth. The morphological and biochemical characteristics of endophytic bacteria isolated from Cymbopogon citratus were studied. Antibacterial activity was studied by the disc diffusion method with known antibiotic ciprofloxacin (CIP) as standard. Twenty bacterial isolates from leaves of Cymbopogon citratus were isolated. The microscopic examination of endophytic bacteria showed that isolates from leaves of Cymbopogon citratus were gram positive rods. The biochemical characterization of endophytic bacterial isolates from Cymbopogon citratus showed positive reaction to catalase and oxidase and negative reaction to coagulase test. All the isolates had shown negative reaction to various enzymic activity tests. Endophytic bacteria from leaves of Cymbopogon citratus had shown antibacterial activity against Staphylococcus aureus and Bacillus cereus.