Vol. 8, Issue 9 (2019)

Author(s):
Rocky Thokchom, Soumen Maitra, Bikash Bhattarai and RK Imotomba

Abstract:
The growth of in-vitro regenerated callus and well rooted plantlets from Japanese Iris leaf tissues was analyzed biochemically. Since enzymes are known as metabolic markers, it changes during development and differentiation. Certain proteins and enzymes are responsible for callus proliferation and differentiation into shoot buds during morphogenesis. In the experiment conducted for two consecutive years, MS basal medium supplemented with 2,4-D (1-2 mg/l) and TDZ (0.1 mg/l) produced the maximum protein, peroxidase and polyphenol oxidase contents in the 30 days old calli. In the well in-vitro regenerated plantlets, maximum protein content was observed in the basal medium containing only IBA (3 mg/l), while the combination of both IBA (1-2 mg/l) and NAA (1.0 mg/l) yielded higher peroxidase content and polyphenol oxidase content.