Vol. 8, Issue 9 (2019)

Author(s):
B Rajeswari, Dr. N Saritha and Dr. N Devanna

Abstract:
A simple, accurate, precise and robust RP-HPLC method was developed for the simultaneous estimation of the Segesterone and Ethinyl Estradiol in bulk and its pharmaceutical dosage form. Chromatographic separation was achieved on Phenomenex C₁₈ Column (150 x 4.6 mm, 5m). The optimized mobile phase consist of water and acetonitrile in the ratio 45:55%v/v was pumped through column at a flow rate of 0.7 ml/min. Temperature was maintained at 30 °C. Optimized wavelength selected was 260 nm. Retention time of Segesterone and Ethinyl Estradiol were found to be 2.110 min and 2.654min. %RSD of the Segesterone and Ethinyl Estradiol were and found to be 0.9 and 0.7 respectively. %Recovery was obtained as 99.91% and 98.61% for Segesterone and Ethinyl Estradiol respectively. LOD, LOQ values obtained from regression equations of Segesterone and Ethinyl Estradiol were 0.11, 0.32and 0.05, 0.15 respectively. Regression equation of Ethinyl Estradiol is y = 21000x + 637.6 and y = 25485x + 24862 of Segesterone. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.