Pathology and molecular diagnosis of infectious bursal disease in an organized farm
Rahmaan SB, Sajitha IS, Dhanush Krishna B, K Krithiga, Surya Sankar, Dhivahar M and Mammen J Abraham
The present investigation was conducted to find out the cause of huge mortality in University Poultry and Duck Farm, Mannuthy. About sixty poultry carcasses presented to the Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy formed the materials for the study. On detailed postmortem examination, enlargement of bursa of Fabricius with presence of mucous exudate in lumen, haemorrhages in thigh muscles and swollen kidneys were noticed. On microscopical examination, bursa of Fabricius revealed severe lymphoid depletion, shrinkage of follicles and accumulation of necrotic debris in follicles. Lesions were suggestive of infectious bursal disease. Etiological confirmation of the cases were done with a reverse transcriptase polymerase chain reaction (RT-PCR) targeting the VP2 gene of Infectious bursal disease virus (IBDV), which revealed an amplicon size of 480 bp. Molecular and pathological findings with bursal lesions confirmed the disease as infectious bursal disease.
How to cite this article:
Rahmaan SB, Sajitha IS, Dhanush Krishna B, K Krithiga, Surya Sankar, Dhivahar M, Mammen J Abraham. Pathology and molecular diagnosis of infectious bursal disease in an organized farm. Pharma Innovation 2019;8(9):78-81.