Vol. 8, Issue 9 (2019)

Author(s):
Amrutha TA, AK Beena, Aparna Sudhakaran V, Rejeesh R, Archana C and Vinod V

Abstract:
The objective of this study was to evaluate the functional properties of a lactic acid bacteria isolated from tender coconut water. On the basis of biochemical characteristics and 16S rRNA sequencing, the isolate was identified as Weissella cibaria, the sequence of which is deposited in NCBI with accession number MH782084. Acid tolerance, bile tolerance and adhesion potential were assessed in vitro. Weissella cibaria DMA 18 strain was found to withstand pH 3.0 and 0.6% (w/v) concentration of bile salt environment for a period of 3h. Auto-aggregation and cell surface hydrophobicity of 83.78% and 89.1% is suggestive of the good adhesion potential of the isolate. The non-haemolytic nature of the strain indicates the possible absence of virulence factors. Both the culture and crude EPS displayed remarkable antioxidant DPPH assay revealed antioxidant activity of 27% and 33.55% respectively with sample volumes as low as 10 µl and 6µl respectively. The results confirm the potential of Weissella cibaria DMA 18 to be used as a functional culture for designing foods to ameliorate disorders induced by oxidative stress.