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Vol. 8, Issue 8 (2019)

RP-HPLC method development and validation for the simultaneous estimation of glucosamine, methyl Sulfonyl methane and Diacerein in tablet Dosage form

Author(s):
Rajanala Rajeswari, Dr. Devanaboyina Narendra, Yandamuri Narayudu, Penumantra Venkata Kishore and Gadi Vijaya lakshmi
Abstract:
A simple, accurate, precise method was developed for the simultaneous estimation of the Glucosamine Sulphate (GLU), Methyl Sulfonyl Methane (MET) and Diacerein (DIA) in solid dosage form. Chromatogram was run through Symmetry C18 150x4.6mm, 5m. Mobile phase containing 0.01N KH2PO4 buffer (pH-3.5) and Acetonitrile in the ratio of 60:40 v/v was pumped through column at a flow rate of 0.7ml/min. Temperature was maintained at 30 °C. Optimized wavelength for Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein was found to be 210.0 nm. Retention time of Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein were found to be 2.121 min, 2.609 min and 3.576 min respectively. % RSD of system precision for Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein. Were and found to be 0.6, 0.4 and 0.8 respectively. % RSD of method precision for Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein. Were and found to be 0.2, 0.3 and 0.6 respectively. % recovery was obtained as 100.01%, 100.10% and 100.59% for Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein Respectively. LOD, LOQ values are obtained from regression equations of Glucosamine Sulphate, Methyl Sulfonyl Methane and Diacerein were 2.23 µg, 0.51 µg, 0.04 µg and 6.77 µg, 1.54 µg, 0.13 µg respectively. Regression equation of Glucosamine Sulphate was y = 11709x – 11390, Methyl Sulfonyl Methane was y = 3178x – 6144 and of Diacerein was y = 10040x - 1209.6.
Pages: 63-68  |  1402 Views  632 Downloads


The Pharma Innovation Journal
How to cite this article:
Rajanala Rajeswari, Dr. Devanaboyina Narendra, Yandamuri Narayudu, Penumantra Venkata Kishore, Gadi Vijaya lakshmi. RP-HPLC method development and validation for the simultaneous estimation of glucosamine, methyl Sulfonyl methane and Diacerein in tablet Dosage form. Pharma Innovation 2019;8(8):63-68.

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