Vol. 8, Issue 8 (2019)

Author(s):
K Harish, A Jagadeesh Babu, T Madhava Rao and B Sreedevi

Abstract:
Out of 400 milk samples collected from different sources, 374 samples were positive for Staphylococcus aureus by culture method and confirmed by biochemical tests and 191 isolates (51.07%) were positive by PCR method. All the coagulase positive S. aureus isolates (191) were positive for nuc gene. Out of 191 S. aureus isolates, 15.2% isolates were positive for mecA gene. Among the 374 isolates 191 isolates were confirmed as pathogenic S. aureus by a positive coagulase test. A pannel of 8 antibiotic discs were tested by using the standard disc diffusion method. Among the 191 isolates maximum resistance was observed for penicillin (74.9%) followed by gentamycin (27.2%), erythromycin (21%), cefoxitin (15.2%), and ciprofloxacin (11.5%), tetracycline (7.8%), cotrimoxazole (5.8%), chloramphenicol (0.5%).