Vol. 8, Issue 7 (2019)

Author(s):
Deepa Pathipati, CH Srinivasa Prasad, AVN Siva Kumar, B Punya Kumari, RV Suresh Kumar, B Rambabu Naik and VH Rao

Abstract:
The study was conducted to evaluate the effects of co-culture of preantral follicles with cumulus cells or granulosa cells for six days. Preantral follicles (PFs’) were isolated from the ovarian cortical slices using micro dissection method under stereo zoom microscope. The cumulus cells were obtained by repeated pipetting of cumulus oocyte complexes from medium and large sized follicles on surface of the ovary and granulosa cells were aspirated from the oocytectomized preantral follicles. Isolated PFs’ were cultured in TCM 199B which serves as control group or in standard culture medium (TCM 199B supplemented with FSH (2.5 µg/mL), IGF-I(10 ng/mL), GH(1 mIU/mL) and T₄(1 µg/mL). PFs’ were co-cultured with cumulus or granulosa cells in TCM199B and/ standard medium (T3-T6) for six days. Our results indicated that co-culture of PFs’ with Cumulus cells in TCM 199B (T3) yielded better growth and development of preantral follicles in vitro when compared to others.