Vol. 8, Issue 7 (2019)

Author(s):
D Sravanthi, P Vijayalakshmi and P Thiruselvame

Abstract:
A case control study was conducted at small animal medicine unit of Teaching Veterinary clinical complex, Rajiv Gandhi college of Veterinary and Animal Sciences, Puducherry for the period of 3 months. Total of nine dogs exhibiting signs of vomiting and diarrhoea that were brought to the hospital were utilized for the study. Nine age-matched dogs brought to the clinic for vaccination / routine checkup that were apparently normal were taken as a control group. The disease was predominantly noticed in dogs between 0 to 6 months old. The hematological study that was conducted showed a reduction in number of thrombocytes of disease group (1.34±0.152) when compared to a control group (5.26±2.21) due to thrombocytopenia which is a good indicator for prognosis. There was a reduction in total protein and albumin in the disease group (4.74±0.68 and 2.86±1.67) compared to the control group (6.52±0.74 and 3.96±0.48) due to exudative type or permeability diarrhoea. Nine suspected samples were positive using H primer pair generated PCR product of 630 bp. In this study PCR assay was found to be sensitive, specific and rapid technique for detecting CPV infection. The primer pair Hfor/Hrev (Buonavoglia et al., 2001) amplified 630bp product of VP2 gene. The higher sensitivity of PCR assay could be due to its ability to detect low level of virus (10³ PFU / g of faeces).