Vol. 8, Issue 6 (2019)

Author(s):
Balaji Ambore, Avinash Rode, Narayan Dakshinkar, Nitin Kurkure, Vijay Ingle and Meera Sakhare

Abstract:
Oxalate rich plants may become important forage resources for ruminants when other feedstuffs are in short supply. Oxalobacter formigenes is considered as the main oxalate-degrading bacteria in ruminants. The identification and quantification of these bacteria is essential in animals fed with oxalate rich plants. The faecal samples from the animals fed on soybean crop residue and healthy control animals which were not fed on soybean crop residue were collected in a sterile bottle and subjected for their molecular analysis for identification and relative quantification of Oxalobacter formigenes. The genomic DNA was extracted from capsule containing Oxalobacter formigenes 700 million by using PowerFecal DNA isolation kit (MoBio Laboratories, Inc.) for standardization of PCR. The DNAs’ from collected faecal samples of animals fed on SCR were extracted as per the experimental protocol supplied by MoBio Laboratories with slight modifications as per the necessity. Out of 26 faecal samples of SCR fed animals, 15 (57.69%) were positive and 11 (42.31%) were negative for Oxalobacter formigenes while out of 12 control samples (SCR not fed) 3 samples (25.00%) were positive and 9 (75.00%) were negative for Oxalobacter formigenes. Overall 18 faecal samples (47.37%) were expressed positivity for Oxalobacter formigenes. The results for expression of frequency of Oxalobacter formigenes revealed higher frequency of oxc gene in the faecal samples of animals fed with SCR than that of healthy control animals. Relative quantification of O. formigenes revealed high Ct value (28.45 and 29.52)in oxalate toxicityand SCR not fed animals (ranged from 29.16 to 33.28) than SCR fed (ranged from 20.85 to 27.10) and positive control (18.78) indicated more quantity of O. formigenes in the faecal samples of animals fed with SCR than SCR not fed and animals with oxalate toxicity.