Vol. 8, Issue 6 (2019)

Author(s):
Jyothsna Modugula, Sushma Rachamalla, Manohar Reddy Jakkireddy, Indira Devi P, Venkata Seshagiri Podili and Krishnaveni Kamepalli

Abstract:
A simple, economic, selective and precise RP-HPLC method has been developed and validated for Valsartan and Hydrochlorothiazidein bulk and tablet dosage form. The isocratic LC analysis was performed on Inertsil BDS C18 column (250 mm x 4.6 mm, 5 µ) using mobile phase composed of Methanol and 0.05M phosphate buffer pH 4.5 (55:45 v/v) at a flow rate of 1.0 ml/min. Quantitation was performed using UV detector at 281 nm. The retention time was found to be 3.541min for Valsartan and 5.221min for Hydrochlorothiazide. The analytical method was validated according to ICH guidelines. The linearity was observed in the range of 20-60 µg/ml for both Valsartan and 99.67 to 99.94 % for Hydrochlorothiazide with correlation coefficients (r²) are 0.999 for Valsartan and 0.998 for Hydrochlorothiazide. The % recovery was found to be 100.12 - 100.89 % for Valsartan and Hydrochlorothiazide. The relative standard deviation values for repeatability and intermediate precision studies were less than 2%. The proposed method was precise, rapid, accurate, and cost-effective and can be used for the routine estimation for Valsartan and Hydrochlorothiazidein tablet dosage form.