Vol. 8, Issue 6 (2019)

Background: Diabetes mellitus is a clinical syndrome characterised by hyperglycaemia caused by absolute or relative deficiency of insulin. In Ayurveda disease type 2 diabetes can be correlated with Madhumeha. Madhumeha (Type 2 Diabetes) is caused by the involvement of all Doshas and ten Dushya. Nisha Amalaki is one of the Ayurvedic formulation widely used in the management of Madhumeha (Diabetes Mellitus). In the present study 7 Bhavna (trituration) of Amlaki (Emblica officinalis) Swaras (juice) have been given to Haridra Choorna (Curcuma longa).

Aim: To carried out study of Amalakibhavita Nisha with respect to its stability against microbial contamination

Materials and Methods: Sample of Amalakibhavita Nisha was prepared and studied at regular time intervals to check microbial contamination. Vati was stored in plastic container during different climatic conditions were studied at regular intervals of 1 month for a period of 15 months to analysis Mycological findings and presence of bacteriological findings by Wet mount preparation and Gram stain test respectively.

Results: Sample was subjected to the microbiological study from the date of the preparation (17 January 2017) to the date of last microbiological study (30 April 2018). No any contaminations were found in microbiological study.

Discussion: This study was carried out to observe the stability study of Amalakibhavita Nisha store in different climatic conditions and temperature with respect to Microbial Contamination of prepared sample. Thus a baseline Microbial profile was studied at regular interval of 1 month for total 15 month (i.e. time for consumption of prepared drug). At the end of study sample was not showed presence of any Microbes.

Conclusion: At the end of study Vati container has not present of microbes even in different climate and temperature, after 15 months of preparation sample, Hence in present study the stability test of Amalakibhavita Nisha with respect to microbiological findings was negative at room temperature, warm and cold, dry and humid conditions.