Vol. 8, Issue 6 (2019)

Author(s):
Amit Kumar, Jitendra Singh Mehta, Govind Narayan Purohit, Ashok Kumar and Harvindra Kumar Narula

Abstract:
The objective of this study was to determine the effect of combination of vitamin E with selenium (Se) and vitamin C injections on serum total protein and its fractions in Magra rams. Twenty one Magra rams were randomly divided in three groups. The 1^st group (n=7) was administered injection of normal saline (without antioxidant administration) 1ml SC once in a week as a placebo and was considered as a control (C). The rams of group 2^nd (n=7) were given injections of selenium and vitamin E (1.5 mg sodium selenite and tocopherol 50mg/ml) 1ml SC per animal once in a week and considered as group VES. The rams of group 3^rd (n=7) were given vitamin C (250mg sodium ascorbate/ml), 8 ml SC per animal on alternate days and considered as group VC. All the rams received the treatments for 45 days. Blood was collected twice in a week one month post-treatment for 5 consecutive weeks and serum was analyzed for serum total proteins and its fractions like albumin and globulin. Statistical analysis revealed significant (P<0.05) increase in concentration of serum total protein, albumin and globulin whereas A/G ratio remained unaffected by the treatment. The results of this experiment confirm a clear positive effect of parenteral administration of vitamin E combined with Se and vitamin C on serum total proteins and its fractions in rams.