Vol. 8, Issue 6 (2019)

Author(s):
D Raju, P Karunakar, China Babu Jonnakuti and N Asha

Abstract:
The estmation of Metformin hydrochloride and Vildagliptin by simultaneous method using RP-HPLC method was performed by using Chromosil ODS C₁₈ column having 250 x 4.6mm 5μ, and mobile phase containing 0.1M Di Potassium hydrogen phosphate and Methanol at the ratio (60:40%v/v) adjust the pH:9.2 by using Ortho phosphoric acid. The flow rate was 0.5ml/min and detection was observed at 258nm. The retention time of Metformin and Vildagliptin was 1.43min and 5.32min respectively. The all validation parameters were accepted within the limits as per ICH guidelines. The specificity was performed under stress conditions like acid, base, peroxide, light and heat and observed the degradation of drugs. It was found to be within the acceptance limit. Hence it was concluded that the developed method was found to be simple, precise, accurate and rapid method for the routine analysis of Vildagliptin and Metformin hydrochloride API and pharmaceutical dosage form in laboratories.