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Vol. 8, Issue 6 (2019)

Simultaneous estimation of vildagliptin and metformin hydrochloride by using RP-HPLC in bulk and pharmaceutical dosage form

Author(s):
D Raju, P Karunakar, China Babu Jonnakuti and N Asha
Abstract:
The estmation of Metformin hydrochloride and Vildagliptin by simultaneous method using RP-HPLC method was performed by using Chromosil ODS C18 column having 250 x 4.6mm 5μ, and mobile phase containing 0.1M Di Potassium hydrogen phosphate and Methanol at the ratio (60:40%v/v) adjust the pH:9.2 by using Ortho phosphoric acid. The flow rate was 0.5ml/min and detection was observed at 258nm. The retention time of Metformin and Vildagliptin was 1.43min and 5.32min respectively. The all validation parameters were accepted within the limits as per ICH guidelines. The specificity was performed under stress conditions like acid, base, peroxide, light and heat and observed the degradation of drugs. It was found to be within the acceptance limit. Hence it was concluded that the developed method was found to be simple, precise, accurate and rapid method for the routine analysis of Vildagliptin and Metformin hydrochloride API and pharmaceutical dosage form in laboratories.
Pages: 296-301  |  966 Views  685 Downloads


The Pharma Innovation Journal
How to cite this article:
D Raju, P Karunakar, China Babu Jonnakuti, N Asha. Simultaneous estimation of vildagliptin and metformin hydrochloride by using RP-HPLC in bulk and pharmaceutical dosage form. Pharma Innovation 2019;8(6):296-301.

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