Vol. 8, Issue 6 (2019)

Author(s):
Nikita Patel, Dr. Rakesh Jat and Dr. Chirag Desai

Abstract:
A specific, accurate, precise and robust HPTLC method was developed and validated for estimation of Methocarbamol and Diclofenac sodium in combined dosage form. For HPTLC, Hamilton microlitre syringe (Linomat syringe 659.0014, Hamilton-Bonaduz Schweiz, Camag, Switzerland) was used. The solid phase containing cylinder specifications are given bellow here. UV chamber (Camag, Switzerland), Twin trough chamber (20 × 10 cm; Camag, Switzerland), Linomat 5 sample applicator (Camag, Switzerland) and TLC scanner 4 (Camag, Switzerland) operated by win CATS version 1.4.6 software (Camag, Switzerland) were used in the study. All drugs and chemicals were weighed on an electronic balance (AUW 220, Shimadzu Corp., Japan). All data calculations were performed using Microsoft Excel 2010 software (Microsoft Corporation, USA).