Screening of hydrocarbon degrading bacteria isolated from oil contaminated soil
Veerapagu M, Jeya KR, Kalaivani R, Jeyanthi KA and Geethanjali S
The use of indigenous microorganisms in the bioremediation of hydrocarbon pollutants to cleanup environment has become a valuable technique. The aim of the present study was to isolate bacteria from contaminated soil of motor vehicle workshop Perambalur. A total of eleven bacteria isolated was investigated for hydrocarbon tolerance in Bushnell Haas broth containing 1% (w/v) crude oil as sole carbon source. Four bacterial isolates exhibited growth of > 1.0 OD screened for hydrocarbon degradation by DCPIP method. The isolate HDB5 showed 27.5% of biodegradation was identified as Pseudomonas sp and investigated for biodegradation of petrol, diesel and engine oil by gravimetric method for 30 days of incubation revealed 76%, 83% and 69% of degradation. The Pseudomonas sp. isolated could be a potential candidate for the degradation of polycyclic aromatic hydrocarbons.
How to cite this article:
Veerapagu M, Jeya KR, Kalaivani R, Jeyanthi KA, Geethanjali S. Screening of hydrocarbon degrading bacteria isolated from oil contaminated soil. Pharma Innovation 2019;8(6):69-72.