Vol. 8, Issue 6 (2019)

Author(s):
V Prasastha Ram, L Venkateswara Rao, T Srinivasa Rao, KV Subramanyam and K Srinivas

Abstract:
In the present study, a total of 507 samples comprising food of animal origin (215), faecal swab samples (188), human urine samples (65), human diarrhoeic stool samples (12) as well as water samples (27) were examined. Overall prevalence of P. mirabilis was found to be 34.51% (175/507) by species-specific PCR. Among foods of animal origin, the highest rate of P. mirabilis isolates were recovered from chicken samples (38.7%), followed by pork (37.5%) and mutton samples (28.9%). Among faecal swabs, the highest rate of P. mirabilis isolates were from poultry (49%), followed by pigs (37.8%). Human urine samples showed a prevalence rate of 10.7%. Water samples showed 7.4% prevalence. All the human diarrhoeic stool samples were negative for P. mirabilis. Some of the virulence factors genes were investigated using polymerase chain reaction technique. The genes ureC (80.5%), ureA (72.5%), flaA (28.5%), hpmA (60.5%) and zapA (50.28%) were detected in P. mirabilis isolates.