Vol. 8, Issue 5 (2019)

Author(s):
Niren Majumdar and Nakul Chandra Mandal

Abstract:
Colletotrichum gloeosporioides is one of the most common fungal pathogen among different pathogens of banana which remains at quiescent state before maturity and rotting of fruits starts along with the ripening as it becomes active from quiescent state. Twelve different culture media were considered and significant variation in radial growth, sporulation and spore size of the fungus was noticed. The pathogen showed maximum radial growth on Potato Dextrose Agar (87.67mm), Malt extract Peptone Dextrose Agar (83.00mm) and Potato Sucrose Agar (81.33mm) media followed by Malt Extract Agar (79.00mm) and Yeast extract Dextrose Agar (76.67mm) whereas least growth observed on Czapek’s Dox Agar (28.67mm). Highest sporulation recorded on Oat Meal Agar (26.26 X 106 and 45.70 X 106) irrespective of incubation period followed by PDA (24.66 x 106). No sporulation was noticed up to 7 days on Sabouraud’s Agar (SA). Spore size also significantly varies among the media with longest spore on Yeast extract Dextrose Agar (16.64µm) and PSA (16.51µm). The breadth of the spore was more on Water Agar (5.08 µm), Glucose Peptone Agar (4.95 µm) and MPDA (4.95µm) as compared to others. Synthetic media poorly supported growth and sporulation of the pathogen as compare to natural and semi-synthetic media.