Vol. 8, Issue 5 (2019)

Author(s):
Samuel Girgis, Ahmed Faheem and Amal Elkordy

Abstract:
Bovine serum albumin poly É›-caprolactone nanoparticles were prepared by the double emulsion/solvent evaporation method. The effect of some preparative variables like the molecular weight of the polyester and theortical loading of the protein on the size, zeta poetntial and loading efficiency were investigated. Size wss characterized by photon correlation spectroscopy technique using Malvern zetasizer. Zeta potential is characterized by laser anemomerty technique using Malvern zetasizer. Protein loading efficiency and release rate were characterized by bicinchoninic acid assay using plate reader spectrophotometer. Morphology and size of the particles were shown by scanning electron microscopy. It was concluded that increasing the molecular weight of the poly É›-caprolactone at each bovine serum loading could increase loading efficiency which in turn augmented the nanoparticle size and release rate.