Method development and validation of RP-HPLC method for estimation of luliconazole in marketed formulation (Cream)
Tomal Majumder, Md. Razibul Hasan, Pritam Roy, Ratan Pramanik and Md. Nazmul Hasan
A simple, specific, accurate, precise, rapid, robust and selective stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for assay and validated for quantification of antifungal drug with its excipients in its topical dosage form. The mobile phase has been used for separation consisting of Water: Acetonitrile (60:40). Column used was C18 (4.6 X150 mm, 5µm) with flow rate of 2.0 ml/min. Detection wavelength for Luliconazole was 294 nm. The method has been linear at 60-140% range with r2 0.999. Luliconazole has showed 97-103% recovery. The LOD and LOQ were found to be 0.38µg/ml and 1.06µg/ml respectively. Methanol was used as solvent. The method have been robust under various variation with flow rate, detection wavelength and column oven temperature. Developed method can be used routinely for estimation of drug Luliconazole with its excipients in dosage form and stability sample. The validation of method was carried out as per ICH Guidelines.
How to cite this article:
Tomal Majumder, Md. Razibul Hasan, Pritam Roy, Ratan Pramanik, Md. Nazmul Hasan. Method development and validation of RP-HPLC method for estimation of luliconazole in marketed formulation (Cream). Pharma Innovation 2019;8(5):103-108.