Vol. 8, Issue 4 (2019)

Author(s):
Srilatha T, Venkateshwarlu M, Anitha Devi U and Ugandhar T

Abstract:
An efficient system for somatic embryogenesis was standardized. Immature cotyledonary explants of in vitro grown plants of Indian soybean cultivar (Pk 416). Somatic embryos were induced directly from cotyledon explants on Murashige and Skoog’s (MS) medium fortified with different concentrations of 2,4- dichlorophenoxyacetic acid, (1.0 mg/L to 5.0mg/L), fast growing, greenish nodular callus lines containing somatic embryos were established on initiation medium supplemented with (3.0 mg/L 2.4-D respectively. On subculture of Embryo development and well maturation was achieved on MS medium supplemented with (3.0mg/L 2, 4-D + 1.0mg/L TDZ). The well formed embryos germinated into complete plantlets on MS medium containing (3.0mg/L 2, 4-D+1.0mg/L) TDZ. Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (1.0mg/L–5.0mg/L) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 86%. Plants looked healthy with no visually detectable phenotypic variations.