Ayabirungaraja karpam (ABK) is a synergistic poly herbo- metallic formulation. It consists iron, ferric oxide, Juice of lemon, Juice of birungarajam. ABK is recommended as an effective drug for Anemia, Anti- ageing, and premature grey as per reputed siddha literature. The present study was carried out to characterize the bioactive compound profile of ABK of extract using Gaschromatographie Mass spectrometry (GC-MS) analysis. This experiment revealed the biochemical components of the ethanol extract of ABK with distinctive peak. It is an ideal technique known for its higher resolution spectra of structurally similar compounds. The use of electron ionization in mass spectrometry produces distinctive mass spectral fragmentation pattern enabling mass spectra for unknown to be several against libraries, retention time, molecular formula, molecular weight, chemical structure and area percentage.
The aim of the study is to detect bioactive compound of ABK by GCMS analysis. In like that, the qualitative analysis of ethanol extract of ABK was carried out using GC-MS for the identification of bioactive compound of ABK. The nature and structure of the bioactive compounds were identified by the relative concentrations of various compounds getting eluted as function of retention time. The heights of the peak indicate the relative concentration of the components present in the ABK. The mass spectrometer analyses the compound eluted at different times to identify the nature and structure of the compounds. The large compound fragments into small compounds giving rise to appearance of peaks at different ratios. These mass spectra are finger print of that compound which can be identified from the data library.
It is concluded that the finger prints of the compound were identified 18 new compounds from the NIST library data base with various group of chemicals such as Terpene, Diterpene, Phenol, Coumarin and Fatty acid, Fatty alcohol and Plasticizer. The presence of various bioactive componenets enhance efficacy of ABK apart from the management of IDA.