Vol. 8, Issue 3 (2019)

Method: Baliospermum montanum commonly known as red physic nut, is a plant in the family Euphorbiaceae. Urease inhibition activity assay: 100 microlitre (2mg/ml jack bean urease) +100 microlitres of test compound +0.2 ml of 100 millimolar phosphate buffer and pH 6.8 urea containing 25 millimolar, Incubation for 30min in water bath at 37 ⁰C, 600 microlitres of, 4% sulphuric acid+500microlitres of solution A and solution B, Incubation for 30min in water bath at 37 ⁰C, absorbance at 625nm in UV-Visble spectrophotometer.

Results: Ethanolic extract of Baliospermum montanum shows % inhibition of 71.56 shows significant activity.

Conclusion: In the preliminary jack bean urease inhibitory studies using “Berthelot alkaline phenol- hypochlorite method. Baliospermum montanum have shown a very potential urease inhibitory property compared to other drugs and extracts. For further conformation this study should be carry out in depth to establish mechanism of action of urease inhibition by using in vitro methods. This study helps in developing anti H-pylori drugs which can be adjuvant to the anti-ulcer regimen.