Human granulocytic anaplasmosis (HGA) is a transmissive natural focal disease, which is clinically manifested by acute fever, damage of skin, liver, kidney, the nervous system and hematologic disorders. The purpose of our study was to characterize the regional features of clinical manifestations of human granulocytic anaplasmosis, to reveal the most informative clinical and laboratory signs for the diagnosis of HGA, and co-infection HGA with Lyme disease (LD).
Material: We have examined 498 patients with a suspicion of tick-borne diseases with different clinical manifestations who were on treatment in 2006-2014 in regional infectious diseases hospitals in Lviv and Lutsk in Western Ukraine. For confirm HGA diagnosis, the serum of all patients was tested by ELISA for the presence of an antibody (Ig G) to the HGA agent in diagnostic titles (> 100). At the second stage of confirmation of the diagnosis, we determined the Anaplasma sp. DNA in the blood by PCR.
Results: Cases of human granulocytic anaplasmosis during 2006-2014 were laboratory verified in 60 patients (12.0 ± 1.5) %. Among the 60 laboratory-verified cases of HGA, only 30 (50 ± 6.5)% were detected with monoinfection, cases co-infection HGA-LD were in 28 ((46.7 ± 6.4) %) patients and patients with antibodies to HGA and TBE were detected in (3.3 ± 0.7) % cases. The main clinical manifestations of HGA monoinfection in the western region of Ukraine are systemic intoxication syndrome (85.7%), hepatitis (50%), acute nephropathy (46.4%), changes in the hemogram (leukopenia with neutropenia, thrombocytopenia) in 85.7% of patients.
Conclusions: The cases of human granulocytic anaplasmosis on the western region of Ukraine were detected and laboratory confirmed. The most characteristic manifestations of HGA monoinfection and co-infection HGA-LD were determined.