Vol. 8, Issue 2 (2019)

Author(s):
KS Nataraj, A Srinivasa Rao, KV Nagamani, P Divya and B Renukachandrasekhar

Abstract:
A new, simple, precise, accurate and reproducible RP-HPLC method for Simultaneous estimation of bulk and pharmaceutical formulations. Separation of Metformin, Pioglitazone and Glimepiride was successfully achieve WATERS, C18, 25cmx4.6mm, 5µm or equivalent in an isocratic mode utilizing OPA(ortho phosphoric acid) : Methanol (60:40) at a flow rate of 1.0mL/min and elute was monitored at 273nm, with a retention time of 4.7 minutes for Metformin, 6.4 minutes for Pioglitazone and 9.7 minutes Glimepiride. The method was validated for linearity, accuracy, precision, detection limit, quantification limit, specificity, system suitability and solution stability. Results of all validation parameters were within the limits as per ICH guidelines.