Vol. 8, Issue 2 (2019)
Evaluation of genetic diversity among chickpea (Cicer arietinum) genotypes using PCR based simple sequence repeats markers
Urvashi Vashist, Khazan Singh Boora and Manoj Kumar
To assess genetic diversity a set of 50 germplasm accessions of chickpea (Cicer arietinum) were screened for polymorphism using 102 simple sequence repeat (SSR) primers. All the 102 primers produced amplification with a total number of 219 alleles. One hundred and eighty four alleles were polymorphic whereas 35 monomorphic. The number and size of alleles amplified ranged from 1to 7 and 50 to 1500bp respectively. The PIC values of the primers ranged from 0.20 to 1.00. Primers H1O01, H1O09, H5E05 and P74 produced a unique band in genotypes H91-47, FG-711, H00-256 and CSG-8962 respectively which can be used for genotype identification. Two of the genotypes, HC-1 and HC-3, showed the maximum genetic similarity value of 0.91. HK99-27 and CSG-8962 are most diverse genotypes at a minimum value of similarity coefficient i.e. 0.68. This information may be further utilized in the identification of the genotypes.
How to cite this article:
Urvashi Vashist, Khazan Singh Boora, Manoj Kumar. Evaluation of genetic diversity among chickpea (<em>Cicer arietinum</em>) genotypes using PCR based simple sequence repeats markers. Pharma Innovation 2019;8(2):182-188.