Vol. 8, Issue 2 (2019)

Author(s):
Sushma, Vikas Nehra, Deepika and Pooja Kundu

Abstract:
Study was conducted on total of 25 carcasses including nine kids and sixteen lambs of six month age showing respiratory and digestive disturbances brought to the Department of Veterinary Pathology, LUVAS, Hisar during the period of seven months. The pathological lesions were mainly noticed in intestine, liver, abomasum, mesenteric lymph nodes, spleen, lungs, heart and kidneys. For further differentiation and characterization of the lesions the formalin fixed sections were stained by the various standard special tissues staining techniques viz. Taylor’s modified Brown and Brenn method for Gram’s character of bacterial colonization, PAS-Alcian blue staining for mucosubstances and Pearl’s staining for haemosiderin. In enteritis affected carcasses, intestine revealed goblet cell hyperplasia showing blue coloured mucosubstances with PAS-Alcian blue staining. In cases of pnuemonia, Taylor’s staining, section of the lungs revealed the presence of bright red coloured rod shaped gram negative bacteria. In different sections of spleen with severe hemorrhgaes, Perl’s staining revealed presence of haemosiderosis in red pulp area. These differential staining methods may provide a valuable and cost-effective tool for the diagnostic histopathology and for the researchers in histology.