Vol. 8, Issue 12 (2019)

Author(s):
Suraj SV, Suresh N Nair, Sanis Juliet, Sanjay BM, Saranya C, Muhasin Asaf, Shijin MS, Nisha AR and Anoopraj R

Abstract:
A sensitive and simple reverse phase - high performance liquid chromatographic method has been developed for quantitative analysis of sparfloxacin in rat plasma. The method validation was done with respect to linearity, precision, accuracy and sensitivity. The analysis of sparfloxacin was done on reversed phase C18- column [Luna C18 (2), 5µ, 250 X 4.60 mm] using the mobile phase of 1% aqueous acetic acid: acetonitrile at the ratio of 71: 29% (v/v) with a flow rate of 0.7 mL/min and the oven temperature of 300C and was detected at 280 nm. The method showed the linear response with the correlation coefficient of 0.9992. The mean recovery percentage of 3 different concentration (0.1, 0.5 and 1μg/ml) were found to be more than 87±1.547 %. The precision and accuracy was 2.474 to 8.218 % and 0.0035 to 0.0512. The lower limit of detection and quantification of the method was 0.01 μg/ml and 0.025 μg/ml respectively for sparfloxacin in rat plasma. This method was used analyse the plasma concentration of sparfloxacin, following a single oral administration at the dose rate of 200 mg/kg. The pharmacokinetic analysis of sparfloxacin in rats was fitted well in to two compartment model. The maximum plasma concentration attained by sparfloxacin in rats were 7.351±0.884 μg/ml at 0.755±0.049 h. The half-life, apparent volume of distribution, apparent clearance were 8.379±0.962 h, 13.745±1.576 L/kg, and 3.864±0.195 ml/kg/hr respectively. AUC 0-t and MRT were 45.873±2.082 μg/ml*h and 10.964±1.093 h. sparfloxacin is rapidly absorbed from intestine after oral administration and attained good plasma concentration.