Vol. 8, Issue 12 (2019)

Author(s):
Farzana Beegum MA, Ligimol James, Beena AK, Aparna SV and Aysha CH

Abstract:
Study aimed at assessing suitability of paneer whey as growth medium for production of antimicrobial metabolites by Lb. fermentum MH 782089 against S. aureus. Smaller zone of clearance (ZOC) was exhibited by sterilized paneer whey cell free supernatant (PW-CFS) compared to the control MRS broth cell free supernatant (MRS broth- CFS) when incubated at 37℃ for 48h. As antimicrobial activity exhibited by PW-CFS was lower than MRS broth-CFS attempts were made to increase efficiency of PW as growth medium by supplementing it with different levels of sucrose and dipotassium hydrogen phosphate. PW supplemented with 3% sucrose and 0.3% buffering agent (sPW) exhibited maximum ZOC (22.6±0.5). Production conditions were optimised in terms of incubation temperature and period. Highest antimicrobial activity was exhibited in sPW at 37 ℃ for 72h. Thus sterilized paneer whey can be developed as an alternative for MRS broth as growth medium for the production of antimicrobial metabolites by Lb. fermentum MH 782089.