Vol. 8, Issue 11 (2019)

Author(s):
Anindita Biswas, PW Ramteke and Himanshu Pandey

Abstract:
Drug resistance by bacteria makes treatment procedure expensive, difficult and time consuming. Countries where antibiotic could be purchased without prescription, there situations are deadlier and with the increased rate of travelling throughout the globe multidrug resistant bacteria have reached every nook and corner of civilization. That going to lead us to the age where a simple sore throat might cause life risk, like pre-antibiotic era. In the recent years 2D carbon nano composite based drugs have proved its potential, against microbial infections due to their high pay load, and site specific drug delivery. Novel nano composite: combination of-protein synthesis inhibitor- gentamicin, conjugated with efficient nano carrier- graphene could result better in antibiotic resistance with the improved pharmacokinetics at intended site, against topical infection caused by drug resistant E. coli 1614. This study was primarily based on two stages: preconjugation studies including FTIR analysis. There nine different conjugates of gentamicin loaded graphene have been developed and further solid state characterization showed no significant interaction of the drug with the chosen nano-carrier used in conjugate that would lead to cumulative antibacterial activity of drug and carrier. In second stage was to looking for LD₅₀ determination through colony count method. LD₅₀ determination study concluded that, gentamicin loaded graphene is an excellent antibacterial agent against E. coli 1614 with a LD₅₀ of as low as 9.4 µg/ml.