Vol. 8, Issue 11 (2019)

Author(s):
Vellanki Manvitha, A Gopala Reddy, B Anil Kumar and M Jeevanalatha

Abstract:
The present experiment was carried out to investigate the protective and synergistic effect of Withania somnifera and selenium on chlorpyrifos (CPF) induced alterations in biochemical parameters and histology of kidney and heart in rats. Total of forty two male albino Wistar rats were procured and divided into 7 groups consisting of 6 in each. Group 1- Normal Control. Group 2 - Chlorpyrifos (CPF) (@ 12.5 mg/kg body weight/p.o./day). Group 3 – Withania somnifera (@ 100 mg/kg body weight/p.o./day). Group 4 – Selenium (@ 10 µg/kg body weight/p.o./day). Group 5 – Chlorpyrifos (CPF) (@ 12.5 mg/kg body weight/p.o./day) + Withania somnifera (@ 100 mg/kg body weight/p.o./day). Group 6 – Chlorpyrifos (CPF) (@ 12.5 mg/kg body weight/p.o./day) + Selenium (@ 10 µg/kg body weight/p.o./day). Group 7 – Chlorpyrifos (CPF) (@ 12.5 mg/kg body weight/p.o./day) + Withania somnifera (@ 100 mg/kg body weight/p.o./day) + Selenium (@ 10 µg/kg body weight/p.o./day). The experiment was carried out for a period of 28 days. Group 7 rats revealed a significant (P<0.05) decrease in the mean values of blood urea nitrogen (BUN) and creatinine and rejuvenated the histology of kidney and heart when compared to group 2 rats. These results suggested that the co-administration of Withania somnifera and selenium offered remarkable synergistic protective action against CPF induced alterations in biochemistry and histology.