Vol. 8, Issue 1 (2019)
Physicochemical, qualitative and quantitative determination of secondary metabolites and antioxidant potential of Alocasia macrorrhizos leaf extracts
Santosh Kumar Singh, Jay Ram Patel and Arvind Dangi
Plants have served human beings as a natural source for treatments and therapies from ancient times, amongst them medicinal herbs have gain attention because of its wide use and less side effects. In the recent years plant research has increased throughout the world and a huge amount of evidences have been collected to show immense potential of medicinal plants used in various traditional systems. The aim of the present study was to evaluate physicochemical, qualitative and quantitative phytochemical analysis and in vitro antioxidant activities of leaf of Alocasia macrorrhizos collected from Bhopal region of Madhya Pradesh. The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value etc. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminum chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the leaf was assessed against DPPH assay method using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolics content of leaves ethanolic extract was (9.18mg/100mg), followed by flavonoids (2.30mg/100mg). The activities of ethanolic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 24.44 and 48.58 μg/ml respectively. These studies provided information for standardization and correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.
How to cite this article:
Santosh Kumar Singh, Jay Ram Patel, Arvind Dangi. Physicochemical, qualitative and quantitative determination of secondary metabolites and antioxidant potential of <em>Alocasia macrorrhizos</em> leaf extracts. Pharma Innovation 2019;8(1):399-404.